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KMID : 1011420200250040421
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Journal of Korean Ophthalmic Optics Society
2020 Volume.25 No. 4 p.421 ~ p.426
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Cell Death Derived from Photooxidation of A2E by Irradiating Using Smart Device in Retinal Pigment Epithelial Cells
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Jang Ye-Jin
Ha Jeong-Mi
Park Sang-Il
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Abstract
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Purpose: In this study, we investigated the death of A2E-loaded retinal pigment epithelial cells at different concentrations and smart device light irradiation time of A2E, known as the cause of age-related macular degeneration.
Methods: A2E, a synthetic product of two all-trans-retinal molecules and one ethanolamine, was accumulated in retinal pigment epithelial cells (ARPE-19). The cells were exposed to smart device light to induce photooxidation. Cell death was confirmed after light irradiation at different concentrations of A2E and different light irradiation times. The cell viability induced by the smart device light irradiation was confirmed by MTT assay and immunofluorescence (DAPI staining).
Results: When smart device light irradiated the ARPE-19 cells, cell death was resulted differently according to the A2E concentration and light irradiation time. There was no statistically significant result when the A2E concentration was 2.5 μM. Significant apoptosis was observed at concentrations above 5 μM, whereas 90% apoptosis was observed at 40 μM. Significant cell death was observed after 1.5 h of light irradiation. More than 50% of cells died at 6 h whereas more than 90% at 12 h.
Conclusion: In this study, A2E-loaded retinal pigment epithelial cell death was confirmed according to the light irradiation time of the smart device and the concentration of A2E.
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KEYWORD
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Age-related macular degeneration, Retinal pigment epithelium cell, A2E, Smart device, Apoptosis
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